Investigation of the Effect of Lactobacillus Brevis Bacteria on the Expression of Rel A, IKB, and Casp3 Genes in HT29 Colon Cancer Cells

Aims Studies have shown that probiotic bacteria inhibit the onset and progression of carcinogenesis through different pathways. Our objective in this study was to determine effect on expression growth-related genes Rel A, IKB, Casp3 HT29 colon cancer cells Methods & Materials In study, Lactobacillus brevis were first cultured, after supply media conditioning, they treated cells. The bacterium’s cytotoxic (bacterial T cells) investigated using a microculture tetrazolium test (MTT). DNA extracted from cells, Ladder assay performed. Also, 4′,6-diamidino-2-phenylindole (DAPI) performed show cell apoptosis. After ribonucleic acid (RNA) extraction complementary (cDNA) preparation mechanism bacterium cellular signaling, Cas3 measured real-time polymerase chain reaction (PCR) method. Findings (MTT) showed L. b cells’ proliferation, induce apoptosis these A gene proliferation by increasing IKB expression. (DAPI), ladder following treatment regarding mentioned qualitative changes addition, results increased (P=0.038). Conclusion findings indicate stimulates apoptotic signaling pathway It can be used as new strategy or therapy for treatment.